ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (4): 820-827.doi: 10.11843/j.issn.0366-6964.2016.04.023

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The Histochemical Study of Extracellular Matrix in Plateau Tibetan Sheep Epididymis

LI Cong,YUAN Li-gang*,QU Ya-ling,LU Yu-rong,LU Qi-zhong   

  1. (College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
  • Received:2015-09-10 Online:2016-04-23 Published:2016-04-23

Abstract:

 To explore the distribution of extracellular matrix proteins of epididymis in Plateau Tibetan sheep.The histochemistry of Masson’s,Gomori’s,PAS and AB-PAS(pH 2.5)stainning were used to study the microstructure of epididymis in 7 Plateau Tibetan sheep,also the distribution of the laminin (LN),type IV collagen (Col IV) and heparan sulfate glycoprotein (HSPG) was identified by SP immunohistochemical method.The observations made with the light microscope showed that the epithelium of epididymal ducts were columnar ciliated epithelium and the cauda was more abundant with collagen and reticular fiber than in the caput and the corpus,and as well as the PAS positive reaction in the cauda,but AB-PAS positive reaction showed that the caput and the cauda were stronger than the corpus.In addition,immunostaining analysis appeared that LN was strongly presented in the epididymis,the relative expression of HSPG was lower than LN,and Col IV was weakly expressed,but the distribution of LN and HSPG without statistical differences in epididymis (P>0.05).Taken together,the collagen and reticular fiber were more abundant in cauda than caput and corpus in Plateau Tibetan sheep epididymis,and the intensity of the PAS reaction was companied with the changes of the secretion function of epithelium,the Col IV distribution of ECM proteins was participated in basement membrane transport,but HSPG was related to the blood-epididymis barrier,and the distribution of LN may closely related with the membrane construction and secretion the of other extracellular matrix proteins in epididymal ducts.

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